Anti-SETDB1; clone F0838

Application

Research CategoryEpigenetics & Nuclear Function

Anti-SETDB1, clone F0838, Cat. No. MABE1066, is a highly specific mouse monoclonal antibody that detects SETDB1 and has been tested for use in Western Blotting.

Western Blotting Analysis: A representative lot detected SETDB1 in Western Blotting applications (Matsumura, Y, et. al. (2015). Mol Cell. 60(4):584-96).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in human. SETDB1 is ubiquitously expressed, but a strong expression is observed in liver and testis. It is a histone methyltransferase that specifically trimethylates Histone 3 Lysine 9 (H3K9). H3K9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB1 is reported to inhibit adipogenesis. It has also been shown that H3K4/H3K9me3 bivalent chromatin signature keeps expression of Cebpa and Pparg genes very low. Depletion of SETDB1 can induce adipogenesis in mesenchymal stem cells and in preadipocytes. SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and one MBD domain (aa 611-682). It also has a SET domain (aa 820-1282), a pre-SET (aa 744-817), and a post-SET domain (aa 1291-1307). The pre-SET, SET and post-SET domains are required for its methyltransferase activity. In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster. (Ref.: Matsumura, Y., et al., (2015), Mol. Cell 60(4); 584- 596).

Immunogen

His-tagged recombinant fragment corresponding to 213 amino acids from the N-terminal half of murine Histone-lysine N-methyltransferase SETDB1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in 129/S6/SvEv mouse embryonic stem cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SETDB1 in 129/S6/SvEv mouse embryonic stem cell lysate.

Specificity

Clone F0838 recognizes SETDB1 in murine embryonic stem cells. It targets an epitope with in 213 amino acids from the N-terminal half of isoform 1 and 7.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~160 and 180 kDa observed; 144.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).